The Chinese macaque genome has been edited, and then created five clones to put on experiences
Scientists from the Institute of neuroscience, Chinese Academy of Sciences succeeded in cloning animals with a genome that was modified using CRISPR/Cas9.
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First they got some monkeys with the gene knocked out BMAL1, which triggers the circadian cycle, and then cloned one of them. In the end, had five of her clones. Such a method of manufacturing according to Chinese scientists, will allow, first, to do with their colleagues with fewer laboratory animals, and, secondly, to those with genomes that are best suited for different studies.
Scientists have obtained the final result in two stages. First, they conducted a genome editing in already fertilized embryos of monkeys, breaking into them using CRISPR/Cas9 gene BMAL1.
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This gene encodes a protein that activates gene transcription of other proteins: PER and CRY, which, in turn, are closely linked to circadian rhythms — the accumulation of these proteins occurs during the day, and at night they disperse and the whole cycle repeats every 24 hours. If you disable the gene BMAL1, which starts the cycle, the animals are not sleeping, they aktiviziruyutsya motor activity at night, they feel anxious and depressed.
31 of the 88-mi edited embryos were selected to initiate pregnancies, 8 of them ended in the birth of a healthy monkey. Five monkeys were born nokauti BMAL1, three were carriers of a normal, working gene.